PC712

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-53BP1
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse, human
• Application: —
• Citations: 17

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• does not contain: preservative
• species reactivity: mouse, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:3000-1:5000); Immunofluorescence (1:300-1:500, indirect); Immunoprecipitation (1:300-1:500))
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: unmodified
• Gene Information: human ... TP53(7157)

Description

General description

Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.

Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).

This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).

Immunogen

Human

a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence

Application

Immunoblotting (1:3000-1:5000)

Immunofluorescence (1:300-1:500, indirect)

Immunoprecipitation (1:300-1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)

Other Notes

Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable