antibody form
purified antibody
Quality Level
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, opossum, mouse
isotype
IgG
General description
Purified rabbit polyclonal antibody. Recognizes the ~28 kDa Bcl-x protein.
Recognizes the ~28 kDa Bcl-x protein in doxorubicin-treated MCF-7 cells.
bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs, bcl-xL and bcl-xS. The larger mRNA gives rise to a protein product, bcl-xL, which is similar in size and predicted structure to bcl-2. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2. Of the two isoforms of bcl-x, the long (bcl-xL) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression. Like its homolog bcl-2, the mechanism and extent of bcl-x′s function are still increasing. bcl-xL has been shown to modify the cell′s response to oxidants as well as to participate in resistance to chemotherapeutic agents and radiation.
Immunogen
a synthetic peptide (PWIQENGGWDTFVELY) corresponding to amino acids 201-216 of human Bcl-x
Application
Frozen Sections (5 μg/ml)
Immunoblotting (2.5 μg/ml, chemiluminescence)
Paraffin Sections (2.5 μg/ml, pressure cooker pre-treatment required)
Immunoblotting (2.5 μg/ml, chemiluminescence)
Paraffin Sections (2.5 μg/ml, pressure cooker pre-treatment required)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Negative Control
Brain tissue
Brain tissue
Positive Control
Doxorybicin-treated MCF-7 cells (see comments) or intestinal tissue
Doxorybicin-treated MCF-7 cells (see comments) or intestinal tissue
Other Notes
The immunoblotting application applies to human samples only. Mouse and rat frozen intestinal tissue was fixed with Zamboni′s fixative prior to staining. Only random cells will stain for bcl-x in brain tissue. For MCF-7 cells as a positive control, stimul
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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S Krajewski et al.
Cancer research, 54(21), 5501-5507 (1994-11-01)
The in vivo patterns of bcl-X gene expression were assessed in human and mouse tissues using an immunohistochemical approach. Polyclonal antisera were raised against synthetic peptides corresponding to amino acids 46-66 and 61-79 of the human Bcl-X protein and were
W Fang et al.
Journal of immunology (Baltimore, Md. : 1950), 155(1), 66-75 (1995-07-01)
Developing lymphocytes undergo extensive cell death during selection of the immune repertoire. We investigated the influence of bcl-xL, a member of the bcl-2 family of apoptosis regulatory genes, on apoptosis in a model system for negative selection in the B
M González-García et al.
Development (Cambridge, England), 120(10), 3033-3042 (1994-10-01)
Most examples of cell death in animals are controlled by a genetic program that is activated within the dying cell. The apoptotic process is further regulated by a set of genes that act as repressors of cell death. Of these
L H Boise et al.
Cell, 74(4), 597-608 (1993-08-27)
We report the isolation of bcl-x, a bcl-2-related gene that can function as a bcl-2-independent regulator of programmed cell death (apoptosis). Alternative splicing results in two distinct bcl-x mRNAs. The protein product of the larger mRNA, bcl-xL, is similar in
R Datta et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 6(4), 363-370 (1995-04-01)
Acquired resistance to diverse chemotherapeutic agents has been associated with overexpression of the P-glycoprotein. We have selected human U-937 cells for clones resistant to the cytotoxic agents doxorubicin (U-A20) and vincristine (U-V20). The results demonstrate that P-glycoprotein-positive U-A20 and U-V20
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