MABT64

Anti-Nectin-4/PVRL4 Antibody, clone N4.61

clone N4.61.2, from mouse

• Synonym(s): poliovirus receptor-related 4, Ig superfamily receptor LNIR, nectin 4, poliovirus receptor-related protein 4, nectin-4, Nectin-4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N4.61.2, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NP_112178
• UniProt accession no.: Q96NY8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PVRL4(81607)

Description

General description

Nectin-4 is also known as poliovirus receptor-related protein 4 (PRR4 or PVRL4). Nectins are known for being the receptors for the herpes simplex virus. Nectin-4 is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectin-4 is connected with the actin cytoskeleton through afadin inside the cell. Nectin-4 is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-4 seems to adhere primarily to cells expressing nectin-1 and vice versa.

Immunogen

Recombinant protein corresponding to human Nectin-4.

Application

Anti-Nectin-4/PVRL4 Antibody, clone N4.61 is an antibody against Nectin-4/PVRL4 for use in IH, ELISA & IP.

ELISA Anaysis: A previous lot was used by an independent laboratory in ELISA (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).

Immunoprecipitation Anaysis: A previous lot was used by an independent laboratory in IP (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by Immunohistochemistry in ductal carcinoma tissue.

Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected Nectin-4/PVRL4 in ductal carcinoma tissue.

Target description

55 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ductal carcinoma tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable