MABS485

Anti-Ido1, clone 8G11 Antibody

clone 8G11, from mouse

• Synonym(s): Ido1, Indoleamine 2,3-dioxygenase 1, IDO-1, Indoleamine-pyrrole 2,3-dioxygenase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8G11, monoclonal
• Application: FACS, WB
• Citations: 1

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8G11, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002155
• UniProt accession no.: P14902
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IDO1(3620)

Description

General description

Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

~44 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Recombinant protein corresponding to mouse Ido1.

Application

Anti-Ido1, clone 8G11 Antibody is an antibody against Ido1 for use in Western Blotting, Flow Cytometry.

Western Blotting Analysis: A representative lot detected Ido1 in splenic dendric cells from WT or KO mouse tissue lysate (Oberdan Leo, Universite Libre de Bruxelles).
Flow Cytometry Analysis: A representative lot detected Ido1 in splenic dendric cells from mouse (Oberdan Leo, Universite Libre de Bruxelles).

Biochem/physiol Actions

This antibody does not cross react with Ido2.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in Ido1-Flag tag transfected HEK293T cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ido1 in 10 µg of Ido1-Flag tag transfected HEK293T cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable