MABE148

Anti-HMGB1 Antibody, clone HMG1-5H6

clone HMG1-5H6, from hamster

• Synonym(s): High mobility group protein 1, Sulfoglucuronyl carbohydrate binding protein, high mobility group box 1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: HMG1-5H6, monoclonal
• Application: FACS, IF, WB
• Citations: 1

Properties

• biological source: hamster
• Quality Segment: 100, 300
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: HMG1-5H6, monoclonal
• species reactivity: rat, mouse, human
• technique(s): flow cytometry: suitable, immunofluorescence: suitable, western blot: suitable
• NCBI accession no.: NP_034569
• UniProt accession no.: P09429
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HMGB1(3146)

Description

General description

HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.

~ 30 kDa observed

Immunogen

Recombinant protein corresponding to mouse HMGB1.

Application

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected HMGB1 in NIH/3T3 cells. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)

Flow Cytometry Analysis: A representative lot detected HMGB1 in RAW264.7 cells treated with Cytofix/Cytoperm. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Cell Cycle, DNA Replication & Repair

Use Anti-HMGB1 Antibody, clone HMG1-5H6 (Hamster Monoclonal Antibody) validated in WB, IF, FC to detect HMGB1 also known as High-mobility group box 1.

Physical form

Format: Purified

Protein G

Purified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected HMGB1 on 10 µg of NIH/3T3 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable