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HTSCHEM-1RTA

Sigma-Aldrich

Ready-to-Assay Chem-1 Parental Control Frozen Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.84

product name

Ready-to-Assay Chem-1 Parental Control Frozen Cells, Chem-1 Parental Control Frozen Cells are designed for use as a negative control alongside GPCR-expressing Ready-to-Assays Frozen Cells in the Chem-1 background.

biological source

rat

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

detection method

fluorometric

shipped in

dry ice

General description

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Millipore’s Chem-1 Parental Control Frozen Cells are designed for use as a negative control alongside Millipore’s GPCR-expressing Ready-to-Assay Frozen Cells in the Chem-1 background. Chem-1 cells lack endogenous expression of most GPCRs, although they endogenously express thrombin receptors that may be used to confirm responsiveness of the cells in calcium assays.

Cell Line Description

GPCR Cell Lines
Host cells: Chem-1

Biochem/physiol Actions

Protein Target: Not Applicable
Target Sub-Family: Control

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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