Skip to Content
Merck
All Photos(1)

Documents

HATF02500

Millipore

Immobilon® -NC Nitrocellulose Membrane

100 discs, 25 mm, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

Synonym(s):

Transfer membrane

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
40161507
eCl@ss:
32031602
NACRES:
NB.24

product name

Immobilon®-NC Transfer Membrane, 25 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

material

mixed cellulose esters (MCE) membrane (surfactant-free)
nitrocellulose membrane
plain filter
white filter

Quality Level

400

feature

hydrophilic

manufacturer/tradename

Immobilon®

parameter

4 L/min-cm2 air flow rate
60 mL/min-cm2 water flow rate
75 °C max. temp.

technique(s)

western blot: suitable

filter W × L

25 mm

filter diam.

25 mm

thickness

150 μm

refractive index

n/D 1.51

matrix

Immobilon®

pore size

0.45 μm pore size
79 % porosity

capacity

117 μg/cm2 adsorption capacity (insulin)
 160 μg/cm2 adsorption capacity (BSA)
 259 μg/cm2 adsorption capacity (goat IgG)

bubble point

≥2.1 bar, air with water at 23 °C

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with India ink
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

General description

Biologically inert mixtures of cellulose acetate and cellulose nitrate have made MF-Millipore membrane filters one of the most widely used membranes in analytical and research applications.

MF-Millipore filters without Triton surfactant contain minimum amounts of wetting agent and have a lower water extractable content than standard MF-Millipore filters.

Features & Benefits:
- Versatile filter for biological and environmental monitoring applications
- Available in a range of pore sizes, colored black or white, with or without a gridded surface
- Compatible with ethylene oxide, gamma irradiation, and autoclave sterilization methods
Filter Code: HATF
Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

  • Biological solutions
  • Cell contact
  • Very small volumes requiring surfactant-free surfaces
  • Isolation of virus-like particles in wastewater
  • Microplastics analysis grade water
  • Nucleic acid binding, including eDNA

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H228

Hazard Classifications

Flam. Sol. 1

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service