ABE213
Anti-monomethyl-Histone H3 (Lys79) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H3.1t, H3/g
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
dot blot: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
monomethylation (Lys79)
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity
Predicted to react with multiple vertebrate species due to sequence conservation.
This antibody recognizes Histone H3 methylated at Lys79. Using peptide dot blot analysis slight cross-reactivity with dimethyl-Histone H3 (Lys79) and unmodified Histone H3 (Lys79) may be observed. Using peptide inhibition analysis assay no cross reactivity to these peptides is observed.
Immunogen
Epitope: Methylated at Lys79.
KLH-conjugated linear peptide corresponding to human Histone H3 monmethylated at Lys79.
Application
Dot Blot Analysis: A representative lot detected monomethyl-Histone H3 in Dot Blot.
Peptide Inhibition Analysis: A representative lot blocked monomethyl-Histone H3 in HeLa acid extract.
Immunoprecipitation Analysis: A representative lot detected monomethyl-Histone H3 in HeLa acid extract.
Peptide Inhibition Analysis: A representative lot blocked monomethyl-Histone H3 in HeLa acid extract.
Immunoprecipitation Analysis: A representative lot detected monomethyl-Histone H3 in HeLa acid extract.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-monomethyl-Histone H3 (Lys79) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, PIA, IP to detect monomethyl-Histone H3 (Lys79) also known as H3K79me1, Histone H3 (mono methyl K79).
Quality
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:500 dilution of this antibody detected monomethyl-Histone H3 in HeLa acid extract.
Western Blot Analysis: A 1:500 dilution of this antibody detected monomethyl-Histone H3 in HeLa acid extract.
Target description
~17 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa acid extract.
HeLa acid extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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