218829

Caspase-3 Substrate IX, Fluorogenic

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

• Synonym(s): Caspase-3 Substrate IX, Fluorogenic, (Z-DEVD)₂-Rh 110
• Empirical Formula (Hill Notation): C₇₂H₇₈N₁₀O₂₇
• Molecular Weight: 1515.44
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: DMSO: 1 mg/mL
• fluorescence: λ_ex ~485 nm; λ_em ~535 nm
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable