217695
Cdk1 Inhibitor - CAS 220749-41-7 - Calbiochem
The Cdk1 Inhibitor, also referenced under CAS 220749-41-7, controls the biological activity of Cdk1. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
Synonym(s):
Cdk1 Inhibitor - CAS 220749-41-7 - Calbiochem, 3-(2-Chloro-3-indolylmethylene)-1,3-dihydroindol-2-one
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About This Item
Empirical Formula (Hill Notation):
C17H11ClN2O
CAS Number:
Molecular Weight:
294.74
UNSPSC Code:
12352200
Quality Level
Assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
orange
solubility
DMSO: 200 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
A cell-permeable indolylmethylene-2-indolinone derivative that exhibits potent anti-proliferative properties (IC50 = 2 µM HeLa cells). Acts as a selective, reversible and ATP-competitive inhibitor of Cdk1/cyclin B (IC50 = 5.8 µM). Also inhibits Cdk5 with a IC50 = 25 µM. Binds to the ATP pocket on the Cdk1 active site. Does not affect the activity of GSK-3β even at 100 µM concentrations.
A cell-permeable indolylmethylene-2-indolinone derivative that exhibits potent anti-proliferative properties (IC50 = 2 µM in HeLa cells). Acts as a selective, reversible and ATP-competitive inhibitor of cyclin-dependent kinase-1/cyclin B (IC50 = 5.8 µM for Cdk1 and 25 µM for Cdk5). Does not affect the activity of GSK-3β even at 100 µM concentrations. Binds to the ATP pocket in the Cdk1 active site.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Cdk1
Cdk1
Product competes with ATP.
Reversible: yes
Target IC50: 2 µM for anti-proliferative properties in HeLa cells; 5.8 µM against Cdk1/cyclin B
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Andreani, A., et al. 2000. Anticancer Drug Des.15, 447.
Andreani, A., et al. 1996. Anticancer Res.16, 3585.
Andreani, A., et al. 1996. Anticancer Res.16, 3585.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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Stefanie Schmetsdorf et al.
The European journal of neuroscience, 29(6), 1096-1107 (2009-03-24)
Cyclins and cyclin-dependent kinases (Cdks) are the main components that control the orderly progression through cell cycle. In the mature nervous system, terminally differentiated neurons are permanently withdrawn from cell cycle, as mitotic quiescence is essential for the functional stability
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