217594
Anti-CD44 Rat mAb (A020)
liquid, clone A020, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A020, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
canine, bovine, human, rabbit, porcine, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)
General description
Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.
Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
Immunogen
Human
purified human lymphocyte CD44
Application
ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies (see comments and application references)
Radioimmunoassay (1:5000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies (see comments and application references)
Radioimmunoassay (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.
The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shibnath Ghatak et al.
The Journal of biological chemistry, 280(10), 8875-8883 (2005-01-06)
Hyaluronan is enriched in many types of human cancers, and manipulations of hyaluronan expression or interactions have a major influence on tumor progression in animal models. Increased ErbB2 activity is characteristic of several cancers and is responsible for many aspects
Eun-Ju Chang et al.
Journal of cell science, 120(Pt 1), 166-176 (2006-12-14)
The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappaB ligand (RANKL). The extracellular matrix (ECM) is an important factor influencing cell fate.
Tao Yan et al.
Cell death discovery, 8(1), 193-193 (2022-04-13)
Immunosuppressive tumor microenvironment is a crucial factor that impedes the success of tumor immunotherapy, and tumor-associated macrophages (TAMs) are essential for the formation of tumor immunosuppressive microenvironment. Hyaluronic acid (HA) is highly important brick for glioblastoma microenvironment, but whether it
Daniel S Puperi et al.
Biomacromolecules, 17(5), 1766-1775 (2016-04-28)
Advanced tissue engineered heart valves must be constructed from multiple materials to better mimic the heterogeneity found in the native valve. The trilayered structure of aortic valves provides the ability to open and close consistently over a full human lifetime
Jay J Cao et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 20(1), 30-40 (2004-12-28)
HA activates CD44 to stimulate RANKL expression in bone marrow stromal cells. HA stimulation of RANKL is blocked by anti-CD44 antibody and is absent in cells from CD44(-/-) mice. CD44(-/-) mice exhibit thicker cortical bone and a smaller medullary cavity
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