07-2045

Anti-EHMT1 Antibody (CT)

from rabbit

• Synonym(s): G9a like protein, G9a-like protein 1, H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5, Lysine N-methyltransferase 1D, euchromatic histone methyltransferase 1, euchromatic histone-lysine N-methyltransferase 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_079033
• UniProt accession no.: Q9H9B1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... EHMT1(79813)

Description

General description

EHMT1 (euchromatic histone methyltransferase 1), also known as G9a-like protein (GLP1), is a histone methyltransferase. This protein specifically methylates Lysine 9 of histone H3 in vitro. H3Lys9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is thought to be targeted to histone H3 by different DNA-binding proteins including E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.

Specificity

Not tested in other species.

Immunogen

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human EHMT1.

Application

Anti-EHMT1 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of EHMT1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5 & has been validated in ICC, ELISA & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Chromatin Biology

Western Blot Analysis:
This antibody is reported by an outside laboratory to be suitable for Western blotting using EHMT1-transfected 293 cells.


ELISA:
This antibody is reported by an outside laboratory to be suitable for ELISA.

Quality

Immunocytochemistry:
The antibody detects EHMT1 in HeLa cell nuclei.

Target description

~138 kDa

Physical form

Format: Purified

Protein A chromatography

Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable