Dye content is determined by back calculation from the carbon or nitrogen content, while purity is established by HPLC. The dye content is 60%. The impurities are not identified by either dye content or by methods such as HPLC, and no testing for impurities is conducted in this case. Both dye content and dye purity are determined for this product, with the Specification sheet listing minimum specifications for all lots, and individual Certificates of Analysis reporting lot-specific results. Water content is not measured, and the product is not considered hygroscopic.
302643
Trypan Blue
Dye content 60 %, ≥80% (HPLC)
Synonym(s):
Direct blue 14
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25 G
₪624.00
100 G
₪2,085.00
About This Item
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein:
4360496
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
Quality Level
Assay
≥80% (HPLC)
form
powder
composition
Dye content, 60%
technique(s)
microbe id | staining: suitable
color
dark green to brown, or Brown-Black
mp
>300 °C (lit.)
solubility
H2O: 1 mg/mL
λmax
607 nm
ε (extinction coefficient)
≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
Gene Information
human ... PTPN1(5770) , PTPRF(5792)
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General description
Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.
Application
Biochem/physiol Actions
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
- to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
- to stain plant tissue in order to visualize cell death.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Are the descriptions of Product 302643 as "Trypan Blue - 60% Dye content" and the purity limit of ">= 80.0%" on the Specification sheet and CofA referring to the same thing? If they are, why are they different? If not, should both be considered when aiming to achieve a specific dye concentration? Additionally, if it is 60% dye, what makes up the remaining 40%? Should the significant water content in this product be taken into consideration?
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