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G1007243

HPLC Analysis of Tryptic Peptides from Ovalbumin on SeQuant™ ZIC-HILIC

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: SeQuant ZIC-HILIC, PEEK, 150 x 0.3 mm, 5 μm, 200 Å (1.50481.0001)
mobile phase: [A] 0.25% (v/v) formic acid with Milli-q-water; [B] 0.25% (v/v) formic acid in acetonitrile; (10:90, A:B)
gradient: Initial composition: 90% B and 10% A. Linear gradient from 0-40 min
flow rate: 5 μL/min
detector: MicroMass Ultima-QTOF, Needle voltage: 3.1 kV, Cone voltage: 45V (collision energy: 10V; Scan range: m/z 400-1800; 1.5 s cycle time)
injection: 1 μL of 10 pmole digested sample injected in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    T34 (R)ZVSP(-)
    T11 (R)YPILPEYLQZVK€
    T26 (K)VYLPR(M)
    T16 (K)GLWEK(A)
    T12 (K)ELYR(G)
    T10 (R)LYAEER(Y)
    T20 (R)VASMASEK(M)
    T32 (R)ADHPFLFZ1K(H)
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