G1006458
HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (A777028)
mobile phase: [A], 0.15 M potassium phosphate, monobasic, pH 7.0 with potassium hydroxide; [B] 0.15 M potassium phosphate, monobasic, 0.2 M arginine, pH 7.0 with phosphoric acid (mobile phase is either 100% A or 100% B)
flow rate: 0.3 mL/min
pressure: 1030 psi 100% A (71 bar)
pressure: 1165 psi 100% B (80 bar)
column temp.: 25 °C
detector: UV, 280 nm
injection: 2.5 μL
sample: Waters BEH200 SEC protein standard
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
Legal Information
Analyte
accessory
analytical column
mobile phase component
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