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Supelco

SUPELCOSIL™ SAX1 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL™ SAX1 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L14

manufacturer/tradename

SUPELCOSIL™

extent of labeling

12% carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

matrix

silica gel, spherical particle platform

matrix active group

propyltrimethylammonium phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

anion exchange

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General description

SUPELCOSIL™ SAX1 HPLC Column is typically employed as an anion exchange column in chromatographic separations.
The SUPELCOSIL SAX1 column has a strongly basic quaternary aminopropyl phase and is used for separating anions.

Application

SUPELCOSIL™ SAX1 HPLC Column has been used for the separation of:
  • Organoarsenic compounds in marine algae and in biological materials during their analysis using size-exclusion-anion-exchange (SE-AE) high performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICP-MS).
  • Organoarsenic compounds in edible algae during their analysis using bi-dimensional SE-AE-HPLC combined with dual ICP-MS and electrospray tandem mass spectrometry (ESI-MS/MS).
  • Polypeptides in biological matrices during their analysis using supercritical fluid chromatography (SFC) coupled to MS detector.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
280774-04
280774-03
280774-02
280774-01
280647-04

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Speciation of arsenic in edible algae by bi-dimensional size-exclusion anion exchange HPLC with dual ICP-MS and electrospray MS/MS detection.
McSheehy S and Szpunar J
Journal of Analytical Atomic Spectrometry, 15(1), 79-87 (2000)
Feasibility of supercritical fluid chromatography/mass spectrometry of polypeptides with up to 40-mers.
Zheng J, et al.
Analytical Chemistry, 78(5), 1535-1545 (2006)
Gas and liquid chromatography with inductively coupled plasma mass spectrometry detection for environmental speciation analysis-advances and limitations.
Szpunar J, et al.
Spectrochimica Acta. Part B: Atomic Spectroscopy, 55(7), 779-793 (2000)
Trace element speciation analysis of biomaterials by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection.
Szpunar J.
TrAC, Trends in Analytical Chemistry, 19(2-3), 127-137 (2000)

Protocols

EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA)

EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection

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