ZRB2307

Anti-HIF-1α Antibody, clone 2B14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78 (bHLHe78), HIF-1-alpha, HIF1-alpha, MOP1, Member of PAS protein 1, PAS domain-containing protein 8, PASD8
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2B14, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 92.67 kDa; observed mol wt ~100 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001521
• UniProt accession no.: Q16665
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... HIF1A(3091)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B14 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects HIF-1 . It targets an epitope within 17 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human HIF-1 .

Application

Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate treated with deferoxamine.

A 1:10,000 dilution of this antibody detected HIF-1 in lysate from HeLa cells treated with deferoxamine (260 M, 24 h).
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HIF-1 in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound HIF-1 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HIF-1 in Jurkat cells (-/+ Hypoxia, 4 h).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Hypoxia-inducible factor 1-alpha (UniProt: Q16665; also known as HIF1-alpha, HIF-1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78 (bHLHe78), Member of PAS protein 1, PAS domain-containing protein 8) is encoded by the HIF1A (also known as BHLHE78, MOP1, PASD8) gene (Gene ID: 3091) in human. HIF1- is a subunit of the heterodimeric transcription factor HIF-1, which plays a central role in cellular responses to oxygen deficiency. HIF1- has several important domains: an oxygen-dependent degradation domain (ODD) that regulates its stability, a basic helix-loop-helix (bHLH) domain for DNA binding, and Per-ARNT-Sim (PAS) domains for dimerization with HIF1-β. HIF1- is crucial for the activation of genes that respond to hypoxic conditions. Under normal oxygen levels, HIF1- is rapidly degraded by the proteasome system. In hypoxia, it stabilizes and translocates to the nucleus, where it dimerizes with HIF1-β. The HIF-1 complex then binds to hypoxia-responsive elements (HREs) in the DNA, activating genes involved in angiogenesis, metabolism, iron transport, and cell survival. This response is vital for adapting to low oxygen environments and is crucial in processes like embryonic development and wound healing. Its overactivation can contribute to cancer progression by promoting angiogenesis, metabolic reprogramming, and survival under hypoxic tumor microenvironments. Conversely, its activation can be protective in ischemic diseases like myocardial infarction and stroke by promoting adaptive responses. HIF1- is also implicated in chronic kidney disease, where hypoxia is a key factor in disease progression. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable