Skip to Content
Merck
All Photos(1)

Documents

SML1964

Sigma-Aldrich

NS3728

≥98% (HPLC)

Synonym(s):

1-[3,5-bis(Trifluoromethyl)phenyl]-3-[4-bromo-2-(1H-tetrazol-5-yl)phenyl]urea, Endovion, N-(3,5-bis(Trifluoromethyl)phenyl)-N′-(4-bromo-2-(1H-tetrazol-5-yl)phenyl)urea, NS 3728, NS-3728

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C16H9BrF6N6O
CAS Number:
265646-85-3
Molecular Weight:
495.18
UNSPSC Code:
12352200
NACRES:
NA.77

Assay

≥98% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 10 mg/mL, clear

storage temp.

2-8°C

InChI

1S/C16H9BrF6N6O/c17-9-1-2-11(13-26-28-29-27-13)12(6-9)25-14(30)24-10-4-7(15(18,19)20)3-8(5-10)16(21,22)23/h1-6H,(H2,24,25,30)(H,26,27,28,29)

InChI key

OQRAKHDEGGGWQO-UHFFFAOYSA-N

Biochem/physiol Actions

NS3728 (Endovion) is a di-aryl-urea-based reversible anion channel blocker that is shown to inhibit Ca(2+)-independent Cl- conductance by volume-regulated anion channel (VRAC or VSOAC; IC50 = 400 nM) as well as Ca2+-activated Cl- ccurrent (CaCC) by anoctamin (ANO) in an apparently voltage-dependent manner (IC50 against ANO1 = 2.1 and 0.7 μM at -95 and +50 mV, respectively). NS3728 is reported to be 5.5-times more potent than Tamoxifen against VRAC (IC50 = 0.4 vs. 2.2 μM).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Thomas Kjaer Klausen et al.
Journal of cellular physiology, 210(3), 831-842 (2006-11-18)
Recent evidence implicates the volume-regulated anion current (VRAC) and other anion currents in control or modulation of cell cycle progression; however, the precise involvement of anion channels in this process is unclear. Here, Cl- currents in Ehrlich Lettre Ascites (ELA)
N Hélix et al.
The Journal of membrane biology, 196(2), 83-94 (2004-01-16)
The endogenous volume-regulated anion channel (VRAC) from HEK293 cells was pharmacologically characterized using the whole-cell patch-clamp technique. Under isotonic conditions a small (1.3 nS), Ca(2+)-independent Cl conductance was measured. However, swelling at 75% tonicity activated a VRAC identified as an
Belinda Halling Sørensen et al.
American journal of physiology. Cell physiology, 310(11), C857-C873 (2016-03-18)
The leucine-rich repeat containing 8A (LRRC8A) protein is an essential component of the volume-sensitive organic anion channel (VSOAC), and using pharmacological anion channel inhibitors (NS3728, DIDS) and LRRC8A siRNA we have investigated its role in development of Cisplatin resistance in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service