SAB3701172

Anti-Mouse IgG1 (γ-chain specific)-Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): ALP, Alk Phos
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• concentration: 1.0 mg/mL
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG1 is induced due to antibody responses to membrane proteins and soluble protein antigens. It is the most abundant subclass of IgG.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Alkaline Phosphatase (calf intestine) and Anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Mouse heavy or light chain isotypes.

Immunogen

Mouse IgG1 heavy chain

Application

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit has been used in ELISA.

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable