S6045

Anti-Stem Cell Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• MDL number: MFCD01865052
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): neutralization: suitable
• storage temp.: −20°C
• Gene Information: mouse ... Kitl(17311)

Description

General description

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; the receptors to Stem cell factor (SCF; c-Kit ligand or mast cell growth factor) being the most critical. The signalling triggered by SCF results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by SCF are signalling mediated by Akt, mTOR, MAPK and c-Jun. In addition to development of mast cells, SCF also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
Anti-Stem Cell Factor antibody reacts specifically with mouse stem cell factor.

Specificity

The antibody will neutralize the biological activity of recombinant mouse SCF.

Immunogen

recombinant mouse SCF

Application

Anti-Stem Cell Factor antibody may be used to neutralize SCF at a concentration of 25 ng/ml in 1X10⁵/ml TF-1 cells. The antibody has been used to neutralize SCF in primary murine embryonic fibroblast (MEF) culture. The recommended concentration for indirect ELISA is 1.0 μg/ml and for immunoblotting is 2.0 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Preparation Note

Purified using protein G

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves