RES6008H-A7

HEPES, Hemisodium Salt

• Synonym(s): HEPES hemisodium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt
• Empirical Formula (Hill Notation): C₈H_17.5N₂O₄S· 0.5Na
• CAS Number: 103404-87-1
• Molecular Weight: 249.30
• UNSPSC Code: 12352200
• NACRES: NA.21

Properties

• biological source: synthetic
• Assay: ≥96.0% NaOH basis (Reverse titration)
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin and microbial; tested
• useful pH range: 6.8-8.2
• pKa: 7.5
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

Application

HEPES Hemisodium is a biological buffer often referred to as a “Good′s” buffer. As the hemisodium salt, this buffer can be used directly to prepare a working buffer solution of HEPES. This biological buffer formulation is ideal since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Hemisodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES Hemisodium can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES6008H-A701X: 100 gm container
RES6008H-A702X: 1 kg container
RES6008H-A704X: 10 kg container

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable