indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells
Retinoblastoma-associated binding protein 46 (RbAp46), also referred to as retinoblastoma-binding protein 7 (RBBP7) has 89% amino acid identity with RbAp48. This nuclear protein belongs to the WD-repeat protein family. It has four repeats that ends with Trp-Asp(WD) residues. RbAp46 is an important constituent of the histone modifying and remodeling complexes. RbAp46 gene is located on human chromosome Xp22.13
Specificity
Anti-RbAp46, N-terminal specifically recognizes human RbAp46.
Immunogen
synthetic peptide corresponding to amino acids 1-14 of human RbAp46, conjugated to KLH via a C-terminal added cysteine residue. The sequence differs in mice by one amino acid.
Application
Anti-RbAp46, N-terminal antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in the modification and remodeling of chromatin during cell growth and differentiation. It is associated with the nucleosomal remodeling and deacetylation complex (NURD). It may also play a key role in histone deacetylation and transcription repression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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The role of retinoblastoma-associated proteins 46 and 48 in estrogen receptor alpha mediated gene expression
Creekmore AL, et al.
Molecular and Cellular Endocrinology, 291(1-2), 79-86 (2008)
Characterization of the gene encoding mouse retinoblastoma binding protein-7, a component of chromatin-remodeling complexes
Yang J, et al.
Genomics, 80(4), 407-415 (2002)
Rb-associated protein 46 (RbAp46) inhibits transcriptional transactivation mediated by BRCA1
Chen GC, et al.
Biochemical and Biophysical Research Communications, 284(2), 507-514 (2001)
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