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N9879

Sigma-Aldrich

NADase from porcine brain

≥0.007 unit/mg solid

Synonym(s):

DPN Nucleosidase, NAD+ glycohydrolase

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5 G
€299.00

About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

€299.00


Please contact Customer Service for Availability

Request a Bulk Order

form

powder

specific activity

≥0.007 unit/mg solid

solubility

insoluble

storage temp.

−20°C

General description

NADase is a glycohydrolase that catalyzes ADP-ribose transfer. [1]

Application

NADase from porcine brain has been used in a study to investigate histidine and related compounds resulting from catalyzed ADP-riboslyation. [2] It has also been used in a study to investigate the preparation of arylazide-substituted pyridine adenine dinucleotides for photoaffinity labeling. [3]

Biochem/physiol Actions

Nicotinamide has been shown to inhibit NADase activity. [4]

Unit Definition

One unit will hydrolyze 1.0 μmole of β-NAD to nicotinamide and ADP-ribose per min at pH 7.3 at 37 °C.

Physical form

Acetone-dried powder

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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D San Segundo et al.
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Nature reviews. Drug discovery, 11(11), 822-822 (2012-11-06)
Enzymatic extraction procedure for the liquid chromatographic determination of niacin in foodstuffs.
Ndaw, S., et al
Food Chemistry, 78(1), 129-134 (2002)
H Kim et al.
Molecular and cellular biochemistry, 138(1-2), 237-243 (1994-09-01)
NAD glycohydrolases are the longest known enzymes that catalyze ADP-ribose transfer. The function of these ubiquitous, membrane-bound enzymes has been a long standing puzzle. The NAD glycohydrolases are briefly reviewed in light of the discovery by our laboratory that NAD
Shoko Nakayama et al.
Hematology (Amsterdam, Netherlands), 17(6), 317-320 (2012-11-22)
The accurate determination of cytoplasmic immunoglobulin (cIg) light chain (LC) expression is important to differentiate reactive plasmacytosis from a clonal plasma cell neoplasm such as plasma cell myeloma (PCM). Through retrospective analysis, we studied the cytoplasmic kappa/lambda ratio of CD38-positive

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