As long as the diluted samples maintain a hemoglobin content above the detection limit of the kit (0.2g/dL), the kit should function effectively.When the dilution factor of the samples is known, adjusting the calculated concentration by multiplying it with the dilution factor according to the protocol should yield the correct hemoglobin concentration in the samples.
MAK455
Whole Blood Hemoglobin (Hb) Kit
Sufficient for 250 colorimetric tests
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1 KIT
€398.00
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1 KIT
€398.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
input
blood
detection method
colorimetric
relevant disease(s)
hematological disorder
storage temp.
2-8°C
Related Categories
General description
Hemoglobin (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration.
Application
The Whole Blood Hemoglobin (Hb) Kit may be used for:
- Hematology Research
- Drug Discovery
Features and Benefits
- Fast and Efficient: Get hemoglobin results in just 5 minutes with a quick assay reaction, using only 5 μL of blood, saving time and resources.
- Safe and Environmentally Friendly: Our assay kit utilizes non-hazardous chemistry, eliminating the need for toxic chemicals like potassium cyanide and hexacyanoferrate(III) used in the traditional Drabkin′s method, ensuring safety and environmental responsibility.
- Compatibility with High-Throughput Systems: Effortlessly integrate our kit into high-throughput handling systems for streamlined operations, ensuring both efficiency and accuracy in your laboratory processes.
Suitability
The kit is suitable for the quantitative determination of hemoglobin (Hb) in whole blood.
Principle
Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in research and drug discovery. The Whole Blood Hemoglobin (Hb) Kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at A570 nm, is directly proportional to the hemoglobin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
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Is the use of MAK455 with a diluted whole blood sample suitable?
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What is the upper detection limit of MAK455-1KT Whole Blood Hemoglobin (Hb) Kit?
1 answer-
The equivalent Hemoglobin concentration of the Calibrator, which is 12 g/dL, would be utilized as the kit's upper limit of detection.
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