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Key Documents

MAK345

Sigma-Aldrich

Chymotrypsin Activity Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Chymotrypsin Enzyme Activity Kit

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About This Item

UNSPSC Code:
12352200

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

gastrointestinal diseases

storage temp.

−20°C

General description

Chymotrypsin is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).

Suitability

Suitable for the measurement of chymotrypsin activity in tissue lysate or cultured cells

Principle

The Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


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