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M5904

Sigma-Aldrich

Mineral oil

BioReagent, for molecular biology, light oil

Synonym(s):

light mineral oil

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5 ML
€24.20
500 ML
€105.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12181504
NACRES:
NA.52
grade:
for molecular biology
technique(s):
PCR: suitable

€24.20


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Request a Bulk Order

grade

for molecular biology

Quality Level

product line

BioReagent

form

light oil

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.[1]

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay[2]
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures[3]
  • as a continuous phase to prevent droplet coalescence in emulsification system[4]

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.[5][6][7]

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.[8]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Microfluidic flow-focusing in ac electric fields
Tan SH, et al.
Lab on a chip, 14(6), 1099-1106 (2014)
A quantitative analysis of contractility in active cytoskeletal protein networks
Bendix PM, et al.
Biophysical Journal, 94(8), 3126-3136 (2008)
Construction of a full-length enriched and a 5'-end enriched cDNA library using the oligo-capping method.
Yutaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 221, 73-91 (2003-04-22)
Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated

Questions

1–2 of 2 Questions  
  1. Hello, I´m looking for a mineral oil for sulfur pattern production. The COA of this product says "pass" for sufurous compounds, what does this mean?

    1 answer
    1. The production and preparation of this product are considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.

      Helpful?

  2. Esiste una data di scadenza per il prodotto in questione?

    1 answer
    1. The product specification sheet for this product contains a recommended retest date period of roughly four years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. Please view more information here: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf.

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