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L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with 50 ug/ml kanamycin
Synonym(s):
kanamycin agar plates
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About This Item
UNSPSC Code:
41121800
sterility:
sterile; autoclaved
suitability:
selective for Escherichia coli
selective for coliforms
selective for coliforms
Pricing and availability is not currently available.
Recommended Products
grade
for molecular biology
Quality Level
sterility
sterile; autoclaved
form
plates (10 cm)
plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli
selective for coliforms
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Components
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
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