GE27-9261-01

First-strand cDNA Synthesis Kit

Cytiva 27-9261-01

• Synonym(s): cDNA Synthesis Kit
• UNSPSC Code: 41116006
• NACRES: NA.55

Properties

• manufacturer/tradename: Cytiva 27-9261-01
• color: colorless
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

First-Strand cDNA Synthesis Kit is designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. Primers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice. Two primers are included with the kit. pd(N)6 Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5′-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.

The NotI-(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following first-strand synthesis. The NotI restriction site, located at the 5′-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.

First-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)6 Primer, NotI-(dT)18 Bifunctional Primer, and instruction booklet.

Application

First-strand cDNA synthesis kit has been used to reverse transcribe total RNA into cDNA.[1][2]

Features and Benefits

• For synthesis of single-stranded cDNA prior to amplification by PCR.
• Features preassembled bulk reaction mixes to save time and minimize potential for pipetting errors.
• Allows users to choose primers.
• Includes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids