G5149

Gly-Gln monohydrate

≥97% (HPLC), powder

• Synonym(s): Glycyl-L-glutamine
• Empirical Formula (Hill Notation): C₇H₁₃N₃O₄ · H₂O
• CAS Number: 172669-64-6
• Molecular Weight: 221.21
• MDL number: MFCD00150855
• UNSPSC Code: 12352209
• PubChem Substance ID: 24895200
• NACRES: NA.32

Properties

• product name: Gly-Gln monohydrate, ≥97% (HPLC)
• Assay: ≥97% (HPLC)
• storage temp.: −20°C
• SMILES string: O.NCC(=O)N[C@@H](CCC(N)=O)C(O)=O
• InChI: 1S/C7H13N3O4.H2O/c8-3-6(12)10-4(7(13)14)1-2-5(9)11;/h4H,1-3,8H2,(H2,9,11)(H,10,12)(H,13,14);1H2/t4-;/m0./s1
• InChI key: KLFWVRQACSYLOH-WCCKRBBISA-N

Description

Amino Acid Sequence

Gly-Gln

General description

Glycyl-glutamine (gly-gln) is a dipeptide that is used as a substitute for glutamine in cell culture. Higher cell yields are obtained when this dipeptide is used instead of glutamine, where gly-gln affects substrate utilization and metabolism. Cell growth is higher as the concentrations of this dipeptide remain high, due to lower peptidase affinity.

Glycyl-glutamine is a dipeptide form of L-glutamine. Gly-Gln dipeptide exhibits enhanced solubility and thermostability. Moreover, this compound rapidly hydrolyzes into glutamine and glycine following intravenous infusion. Glycine can protect myocardial cells from ischemic injury by preventing cellular membrane permeability. Furthermore, the supplementation of glycyl-glutamine dipeptide proves advantageous for myocardial preservation in rats, as it enhances cardiocyte energy metabolism and augments ATP and glutathione synthesis.

Application

Gly-Gln monohydrate has been used:

• as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture equine embryos
• as a supplement in DMEM/F-12 to culture cleaved horse embryos
• in the human tubal fluid (HTF) medium to study ammonium accumulation in commercially available embryo culture media during incubation and storage

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves