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FLASC

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit

for cellular ATP determination

Synonym(s):

ATP bioluminescence assay kit

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1 KIT
€476.00

€476.00

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1 KIT
€476.00

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.26

€476.00

Please contact Customer Service for Availability

Quality Level

200

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of adenosine 5′-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded.

Application

The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to determine accurately the level of ATP present in neutrophils isolated from the blood of over 100 human test patients. [1]

Kit Components Only

Product No.
Description
  • FL-AAB Dilution buffer 1 mL/vial
  • FL-AAM Assay mix 1 mL/vial
  • FL-AAS ATP standard 1 mL/vial
  • FL-SAR Somatic cell releasing reagent 1 mL/vial

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCP4458
SLCJ9482
SLCH0845
SLCC0450
SLCC0453

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Questions

1–5 of 5 Questions  

  1. Is any protocol available for this product? What is the working concentration of each solution? Which reagent should be used to prepare dilutions?

    1 answer

    1. Please see the link below to review the product technical bulletin. This document contains the preparation, dilution, and working concentrations of the reagents used: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/399/flascbul.pdf

      Helpful?

  2. Can Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?

    1 answer

    1. We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent.  The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.

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  3. Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    1 answer

    1. In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in.  We have no specific information as to how long ATP will be stable in it.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    1 answer

    1. Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.

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