F7305
Fetal Bovine Serum
Mouse Embryonic Stem Cell Screened, suitable for cell culture, sterile-filtered, USDA Approved
Synonym(s):
FBS, FCS, sera, serum
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About This Item
MDL number:
UNSPSC Code:
12352207
Recommended Products
biological source
bovine fetus
sterility
sterile-filtered
quality
USDA Approved
composition
Hemoglobin, ≤25 mg/dL
technique(s)
cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
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General description
Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting balanced growth-promoting and growth-inhibiting functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.
Most Fetal Bovine Serum (FBS) batches do not support mouse ES cells. Sigma-Aldrich has pre-screened multiple FBS batches to increase your chances of selecting a batch that will perform within your laboratory, saving you time and money.
In addition to our extensive Fetal Bovine Serum performance and quality assays (mycoplasma, sterility, endotoxin, hemoglobin, etc.), we have developed low density, feeder-free assays to assess Fetal Bovine Serum′s support of mouse embryonic stem cells. Only those batches that performed well with mouse ES cells were selected for this product.
Most Fetal Bovine Serum (FBS) batches do not support mouse ES cells. Sigma-Aldrich has pre-screened multiple FBS batches to increase your chances of selecting a batch that will perform within your laboratory, saving you time and money.
In addition to our extensive Fetal Bovine Serum performance and quality assays (mycoplasma, sterility, endotoxin, hemoglobin, etc.), we have developed low density, feeder-free assays to assess Fetal Bovine Serum′s support of mouse embryonic stem cells. Only those batches that performed well with mouse ES cells were selected for this product.
Application
FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Analysis Note
- Endotoxin and hemoglobin tested
- Mouse embryonic stem cell screened
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Li Zhou et al.
Genome biology, 9(7), R119-R119 (2008-07-31)
CD8+ NKT-like cells are naturally occurring but rare T cells that express both T cell and natural killer cell markers. These cells may play key roles in establishing tolerance to self-antigens; however, their mechanism of action and molecular profiles are
Ruth M Risueño et al.
PloS one, 3(3), e1747-e1747 (2008-03-06)
How the T cell antigen receptor (TCR) discriminates between molecularly related peptide/Major Histocompatibility Complex (pMHC) ligands and converts this information into different possible signaling outcomes is still not understood. One current model proposes that strong pMHC ligands, but not weak
Silvia Goldoni et al.
The American journal of pathology, 173(3), 844-855 (2008-08-09)
Decorin, a member of the small leucine-rich proteoglycan gene family, down-regulates members of the ErbB receptor tyrosine kinase family and attenuates their signaling, leading to growth inhibition. We investigated the effects of decorin on the growth of ErbB2-overexpressing mammary carcinoma
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