F1879

Formaldehyde Dehydrogenase from Pseudomonas sp.

lyophilized powder, 1.0-6.0 units/mg solid

• Synonym(s): FDH, Formaldehyde:NAD⁺ oxidoreductase
• CAS Number: 9028-84-6
• Enzyme Commission number: 1.2.1.46 (BRENDA, IUBMB)
• MDL number: MFCD00131100
• UNSPSC Code: 12352204

Properties

• form: lyophilized powder
• specific activity: 1.0-6.0 units/mg solid
• storage temp.: −20°C

Description

General description

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.

Application

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:

• For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
• As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.
• In coupled pectin methyl esterase (PME) enzyme assay.

Biochem/physiol Actions

Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).

Unit Definition

One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing ~70% stabilizers as Mg²⁺, Ca²⁺, bovine serum albumin, glycine, and lysine

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves