This is 10 mM each and can be found under Description-> General Description: The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water.
D7295
Deoxynucleotide Mix, 10 mM
Molecular Biology Reagent
Synonym(s):
Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
Sign Into View Organizational & Contract Pricing
Select a Size
0.2 ML
€73.70
0.5 ML
€163.00
5 ML
€782.00
20 X 0.2 ML
€869.00
All Photos(1)
Select a Size
Change View
0.2 ML
€73.70
0.5 ML
€163.00
5 ML
€782.00
20 X 0.2 ML
€869.00
About This Item
UNSPSC Code:
41106305
NACRES:
NA.52
Recommended Products
Quality Level
form
liquid
concentration
10 mM
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
Application
dNTP Mix has been used:
- in the PCR amplification of genomic DNA isolated from insect,[1] fungi,[2] virus, human,[3]
- in reverse transcription of total RNA to cDNA.[4][4]
- as a component of the DNA amplification mixture for polymerase chain reaction (PCR)[2][5][1]
- routine and long PCR
- manual and automated DNA sequencing
- cDNA synthesis[6] and labeling reactions
Features and Benefits
- Purity of each dNTP: Minimum 99%
- Conveniently formulated; 1 μL is used per 50 μL PCR
- Equimolar amounts of each dNTP means less pipetting
- Minimize risk of contamination in PCR
- UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 8-8 (1989)
Ayokunle O Olanrewaju et al.
Virology journal, 18(1), 77-77 (2021-04-17)
Maintaining adequate drug adherence is crucial to ensure the HIV prevention benefits of pre-exposure prophylaxis (PrEP). We developed an enzymatic assay for rapidly measuring tenofovir-diphosphate (TFV-DP) concentrations-a metabolite that indicates long-term PrEP adherence. The study was conducted at the Madison
Ajay V Maker et al.
Journal of the American College of Surgeons, 228(5), 721-729 (2019-02-23)
Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay
Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
Protocols
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
-
Is this 10 mM each, or 10 mM total? This info should be clearly stated on the label and on your site.
1 answer-
Helpful?
-
-
Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
1 answer-
The product contains Thymidine 5´-triphosphate sodium salt. Thymidine has a deoxy sugar, so it is a deoxythymidine.
Helpful?
-
-
What are the components to Product D7295, Deoxynucleotide Mix?
1 answer-
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water. This is for a total concentration of 40 mM.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
1 answer-
One μl is sufficient for a standard 50 μl PCR reaction.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
