A rat prostate tumour cell line derived from successive in vivo passages of the AT-1 tumour cell line (Sigma Catalogue number. 94101449). The cells are anaplastic, androgenin dependant and metastatic to the lung.
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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