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product name
Clone M-3, 87081806
biological source
mouse skin
growth mode
Adherent
karyotype
Hypertetraploid
morphology
Epithelial
products
Melanin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse (CxDBA)F1 melanoma
Cell Line Description
Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
Application
Cloning studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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