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Key Documents

JW184/2/1

Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl), 87080704

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About This Item

UNSPSC Code:
41106514

biological source

Mouse + Human Transfectoma

ImmunoDon

Not applicable

Myeloma

J558L

Reactivity

Anti-NP (4-hydroxy-3 nitrophenacetyl)

technique(s)

cell culture | mammalian: suitable

isotype

IgG4

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse J558L + Mouse VH gene + Human C (gamma 4) gene

Cell Line Description

This human chimeric antibody is directed against the hapten 4-hydroxy-3 nitrophenacetyl. The V lambda of the endogenous light chain of the plasmacytoma line complements the VNP of the transfected heavy chain to yield the specific antibody. Gene marker: gpt.

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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