As mentioned in the 'DESCRIPTION' section, the composition of this product is considered proprietary and may not be shared. This product does not contain any denaturants except for a proprietary mild detergent. Lysates obtained with this product may be immediately run on a SDS PAGE after the addition of loading buffer.
C2978
CelLytic™ M
Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
Synonym(s):
Whole-cell protein extraction solution
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50 ML
€106.00
250 ML
€374.00
€106.00
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50 ML
€106.00
250 ML
€374.00
About This Item
UNSPSC Code:
41116134
NACRES:
NA.56
€106.00
Please contact Customer Service for Availability
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General description
CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.
Features and Benefits
- Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
- Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
- Convenient: Ready-to-use reagent requires no scraping from culture plates
- Fast: Rapid cell lysis at room temperature.
Quantity
CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).
Analysis Note
Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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Does the CelLytic M (Cat # 2978) contain DTT, and if it does, at what concentration?
1 answer-
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Is the product compatible with the BCA assay? Additionally, are there any denaturants in the formulation?
1 answer-
CelLytic™ M is suitable for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.Helpful?
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Is the C2978 lysis buffer suitable for viral DNA extraction from cultured mammalian cells (fewer than 25,000 cells/ul) and subsequent qPCR?
1 answer-
The cellular DNA is centrifuged down and will not be present in the supernatant along with the proteins. To isolate the DNA, a different kit is necessary.
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What is the critical micelle concentration (CMC) of the product?
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Unfortunately, the CMC value has not been tested for this product.
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Does the CelLytic M (Cat. No.: 2978) contain SDS, and if it does, at what concentration?
1 answer-
The product with the code C2978, CelLytic M, does not contain SDS. Instead, it contains a mild detergent in a buffer.
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Has CelLytic been tested for neutralizing HIV or use in a BSL3 facility?
1 answer-
We have not tested CelLytic for neutralizing HIV nor used in a BSL3 facility.
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Is there any denaturants in Product C2978, CelLytic™ M that can interfere with my downstream applications?
1 answer-
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.
Helpful?
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Will CelLytic™ M, Product No. C2978, interfere with protein concentration assays such as Bradford, Lowry and BCA?
1 answer-
We have tested and found CelLytic™ M to be compatible for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Will Product No. C2978, CelLytic™ M solubilize membrane proteins?
1 answer-
CelLytic™ M can solubilize membrane proteins as well as cytoplasmic proteins and the conditions are non-denaturing. However, we cannot guarantee the solubilization of all membrane proteins. The following proteins were detected in the CelLytic™ M lysate when tested with these antibodies against membrane proteins: Placental alkaline phosphatase, CD45, Connexin and Caveolin.
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