C0897
Citrate Lyase from Klebsiella pneumoniae
lyophilized powder, ≥0.20 unit/mg solid
Synonym(s):
Citrate oxaloacetate lyase
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About This Item
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form
lyophilized powder
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
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Application
Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).
Biochem/physiol Actions
Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).
Unit Definition
One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.
Physical form
Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Isolation of subunits of citrate lyase and characterization of their function in the enzyme complex.
P Dimroth et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3458-3462 (1975-09-01)
Citrate lyase [EC 4.1.3.6; citrate oxaloacetate-lyase (pro-3S-CH2-COO--leads to acetate)] from Klebsiella aerogenes has been dissociated with urea; the three different subunits, alpha-chain (molecular weight congruent to 54,000), beta-chain (molecular weight congruent to 32,000), and gamma-chain (acyl carrier protein; molecular weight
M Suzuki et al.
Archives of microbiology, 175(1), 75-78 (2001-03-29)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected
Julia Gaye-Siessegger et al.
Isotopes in environmental and health studies, 40(3), 181-190 (2004-09-17)
The use of stable isotope techniques for the reconstruction of diets has increased over the last decade. However, isotopic ratios in an animal are not only affected by the composition of the feed, but also by the amount of feed
Citric acid mediates the iron absorption from low molecular weight human milk fractions
Palika R, et al.
Journal of Agricultural and Food Chemistry, 61(46), 11151-11157 (2013)
Celia W Goulding et al.
Journal of molecular biology, 365(2), 275-283 (2006-10-27)
Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should
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