B7661

Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse

clone PTR-8, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Phosphothreonine, Phospho Thr, Phospho Threonine, Phospho-Thr, Phospho-Threonine, p-Thr
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PTR-8, monoclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:40,000 using phosphothreonine-BSA at 10 μg/ml, and using ExtrAvidin-HRP at 2 μg/ml; dot blot: 1:120,000
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containing proteins. Certain proteins known to contain phosphorylated threonine may not be recognized by this antibody due to steric hindrance of the recognition site.

Monoclonal Anti-Phosphothreonine (mouse IgG2b isotype) is derived from the PTR-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.

Immunogen

phosphothreonine conjugated to keyhole limpet hemocyanin (KLH).

Application

Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse has been used in phosphoprotein staining.

Biochem/physiol Actions

Phosphorylation is a rare post-translational event in normal tissue. However, the abundance of phosphorylated cellular proteins increases several-fold following various activation processes. The residues phosphotyrosine, phosphoserine or phosphothreonine (p-Tyr/p-Ser/p-Thr) mediate such processes. Many signal transduction pathways, such as the epidermal growth factor (EGF) and insulin receptor systems, contain Tyr/Ser/Thr kinase which phosphorylates specific Tyr/Ser/Thr residues upon binding of ligands to their receptors. Monoclonal Anti-Phosphothreonine-Biotin may be used as an analytical tool for the identification and quantification of threonine-phosphorylated proteins

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable