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77866

Sigma-Aldrich

Fluorescent IEF-Marker pI 5.5 solution

3 mg/mL in 15 mM HCl

Synonym(s):

IEF-Marker fluorescent pI 5.5

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About This Item

MDL number:
MFCD03099325
UNSPSC Code:
41105336
NACRES:
NA.26

form

liquid

mol wt

Mw 321.27 g/mol

concentration

3 mg/mL in 15 mM HCl

fluorescence

λex 325 nm; λem 412 nm in 0.1 M citrate pH 5.5

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

2-8°C

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General description

filtered through a 0.45 μm membrane filter

Application

suitable as IEF-Marker for HPCE and for IEF-gels

Reconstitution

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1 μl per lane).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Articles

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

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