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60128

Sigma-Aldrich

Potassium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym(s):

KCl

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About This Item

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.26

grade

for molecular biology

product line

BioUltra

Assay

≥99.5% (AT)

form

solid

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.2% loss on drying, 110 °C

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

770 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.98 g/mL at 25 °C (lit.)

anion traces

bromide (Br-): ≤700 mg/kg
iodide (I-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤2 mg/kg
Li: ≤5 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

SMILES string

[Cl-].[K+]

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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Application

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Other Notes

In DpnI/MboI assay for detection of methylation changes in DNA replication

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M.L. DePamphilis
Methods in Enzymology, 262, 653-653 (1995)
Yuanyuan Qu et al.
Nucleic acids research, 41(10), 5263-5272 (2013-04-13)
Bacterial nucleoid-associated proteins, such as H-NS-like proteins in Enterobacteriaceae, are abundant DNA-binding proteins that function in chromosomal DNA organization and gene transcription regulation. The Mycobacterium tuberculosis Lsr2 protein has been proposed to be the first identified H-NS analogue in Gram-positive
Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger
Masatoshi Takagaki et al.
Experimental neurology, 252, 12-17 (2013-11-20)
Sedatives in the neurointensive care unit can strongly influence patients' risks of developing secondary brain damage. In particular, isoflurane, a volatile anesthetic, has been recently re-introduced to the neurointensive care unit, and first clinical studies suggest beneficial effects due to
Hanne Sørup Tastesen et al.
Amino acids, 46(7), 1659-1671 (2014-03-25)
High-protein diets induce alterations in metabolism that may prevent diet-induced obesity. However, little is known as to whether different protein sources consumed at normal levels may affect diet-induced obesity and associated co-morbidities. We fed obesity-prone male C57BL/6J mice high-fat, high-sucrose

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