SAFE Red loading dye is not offered by Millipore Sigma.
S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, SYBR® green gel dye, safer gel stain
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0.5 ML
€556.00
1 ML
€1,030.00
€556.00
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0.5 ML
€556.00
1 ML
€1,030.00
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€556.00
Please contact Customer Service for Availability
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form
solution
Quality Level
usage
1.0 mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
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General description
SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system.[1] SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.[2]
Application
SYBR® Green I nucleic acid gel stain has been used for:
- the quantification of dsDNA[3]
- to stain DNA in polymerase chain reaction (PCR)[4]
- for comet assay technique[5]
- to assess spermatozoon membrane integrity[6]
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[7]
- as a fluorescent dye in flow cytometry[8]
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[8]
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Storage and Stability
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
Legal Information
SYBR is a registered trademark of Life Technologies
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
201.2 °F - closed cup
Flash Point(C)
94 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Does Millipore Sigma offer SAFE Red loading dye?
1 answer-
Helpful?
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What is the molarity of this product?
1 answer-
This product is a 10,000X concentrated solution suitable to prepare 10L of staining solution for in-gel staining of DNA and RNA. The specific concentration is considered proprietary. Published working concentrations for solutions of Sybr Green I range from 0.5 - 1.0 ug/ml. The molecular weight as published by Chebi, is 509.74 g/mol. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/282/803/s9430pis-ms.pdfPlease see the link below to review the Chebi profile:
https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461
chebi file
https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461 MW 509.74
Science direct
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/sybr-green-i
'working' concentration 0.5 - 1.0 ug/mlHelpful?
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Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?
1 answer-
Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine. A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine. This will allow for better staining.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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