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P0290

Sigma-Aldrich

Phenol red solution

0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Phenolsulfonphthalein solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

concentration

0.5%

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

SMILES string

O=S1(C2=CC=CC=C2C(C3=CC=C(O)C=C3)(C4=CC=C(O)C=C4)O1)=O

InChI

1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H

InChI key

BELBBZDIHDAJOR-UHFFFAOYSA-N

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General description

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.

Application

Phenol red solution has been used:
  • as a tracer dye for microinjection of zebrafish eggs
  • as a component of the mMW medium for the preparation of mouse embryos
  • in the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution
  • as a pH indicator in Dulbecco′s modified Eagle medium (DMEM) during glycolytic stress (GST) tests and mitochondrial stress tests (MST) to evaluate metabolic function in cells.

Biochem/physiol Actions

Phenol red solution acts as a pH indicator for the culture medium.Phenol red solution acts as a pH indicator for the culture medium that turns yellow at a pH of 6.4 or lower and changes into red at a pH of 8.2 or higher. It enables a fast and simple assessment of the culture′s health. In addition to its application as a pH indicator in culture mediums, Phenol red is used to detect human peroxidase enzyme activity, as well as cellular hydrogen peroxide levels, in various experiments.

Features and Benefits

High-quality, endotoxin-tested solution, suitable for mammalian cell culture.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Natacha Merindol et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 128, 104423-104423 (2020-05-18)
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR
Shivshankar R Mane et al.
Small (Weinheim an der Bergstrasse, Germany), 14(36), e1801571-e1801571 (2018-08-07)
Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility
Katrin Daniel et al.
Nature communications, 9(1), 3297-3297 (2018-08-19)
The conditional and reversible depletion of proteins by auxin-mediated degradation is a powerful tool to investigate protein functions in cells and whole organisms. However, its wider applications require fusing the auxin-inducible degron (AID) to individual target proteins. Thus, establishing the
Tina Cheng et al.
Micron (Oxford, England : 1993), 129, 102782-102782 (2019-11-28)
Infection of zebrafish with natural pathogen Mycobacterium marinum is a useful surrogate for studying the human granulomatous inflammatory response to infection by Mycobacterium tuberculosis. The adaptive immune system of the adult stage zebrafish offers an advance on the commonly used
Ngoc Tung Tran et al.
STAR protocols, 1(1), 100028-100028 (2020-07-21)
Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse

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