KK3009

KAPA Taq EXtra PCR Kit

• Synonym(s): PCR, Taq
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• usage: sufficient for 500 reactions
• Quality Level: 100
• feature: High Fidelity PCR; Long Range PCR; dNTPs included; hotstart: no
• packaging: pkg of 250 U
• manufacturer/tradename: Roche
• technique(s): PCR: suitable
• input: purified DNA
• storage temp.: −20°C

Description

Application

The KAPA Taq EXtra PCR system is ideally suited for:

• PCR of short- and medium-length targets (<5 kb)
• Long-range PCR (5–15 kb)
• PCR with limiting amounts of template DNA.

Biochem/physiol Actions

The KAPA Taq EXtra PCR system is a blend of KAPA Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support robust, long range, and sensitive PCR. Both enzymes possess 5′→3′ polymerase activity, but only KAPA Taq DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. Reaction products generated with KAPA Taq EXtra are polyA-tailed, and can be cloned into TA-cloning vectors.

Features and Benefits

• KAPA Taq EXtra is a blend of KAPA Taq and a proofreading DNA polymerase.
• Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
• Fidelity is 2-4 times better than Taq
• For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
• Amplify mid- to long-range targets with high yield and sensitivity.
• KAPA Taq EXtra Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl₂ is supplied separately for optimization.
• The fidelity of KAPA Taq EXtra is 2-4 fold higher than that of KAPA Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases, such as KAPA HiFi.

Quality

Each batch of KAPA Taq EXtra DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq EXtra PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Kit Components Only

• KAPA Taq EXtra DNA Polymerase 5 U/µL
• KAPA Taq EXtra Buffer (5X)
• KAPA dNTP Mix 10 mM each
• MgCl₂ 25 mM

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash