10109134001
Roche
RNase
from bovine pancreas
Synonym(s):
Rnase
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About This Item
UNSPSC Code:
12352204
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biological source
bovine pancreas
Quality Level
form
dry powder (prepared from crystallized ribonuclease)
specific activity
~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
packaging
pkg of 500 mg
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Specificity
Heat inactivation: 1 mM EDTA
Application
RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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