For most membranes, the nominal molecular weight limit, also referred to as the molecular weight cut-off, is determined experimentally under a standard set of operating conditions. These analyses typically employ purified globular proteins to serve as markers or indicators of the retention characteristics of an ultrafiltration membrane. An ultrafiltration membrane with a stated nominal molecular weight limit should retain (reject) at least 90% of a globular solute of that molecular weight in daltons. However, for a wider safety margin, the selected cut-off should be well below the molecular weight of the solute to be retained. It is recommended to use a cut-off 2-3 times smaller than the molecular weight of the protein to be concentrated.
UFC5003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa
Synonym(s):
Centrifugal concentrator, 3 kDa MWCO, 0.5 mL sample volume
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8 PKG
€68.60
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€168.00
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€527.00
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€2,260.00
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8 PKG
€68.60
24 PKG
€168.00
96 PKG
€527.00
500 PKG
€2,260.00
About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
material:
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
pore size:
3 kDa MWCO
L:
4.99 cm (1.96 in.)
diam.:
10.8 mm
filtration area:
1 cm2
volume:
0.5 mL
sterility:
non-sterile
Recommended Products
material
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
pore size
3 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95%.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Protocols
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Related Content
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
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What is the margin of error for the MWCO? I read somewhere the RECOMMENDATION is to use a MWCO 2-3x smaller than your target. But I was wondering what’s the standard deviation from 3 kDa?
1 answer-
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Is this product compatible with organic solvents, such as DMSO or DMF?
1 answer-
The Amicon Ultra products are suitable for use with samples containing up to 10% DMSO and 10% DMF. Exposure to 100% DMSO is not recommended. Unfortunately, the compatibility of these devices with 100% DMF has not been determined.
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Can I order the filter only, coming with no tube part?
1 answer-
Unfortunately, no, this product is not available as a stand alone item. The tubes are available separately under the product UFC50VL96.
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Is the 0.5 ml 3KDa Amicon device (UFC5003) compatible with a solution containing 5% SDS (Sodium Dodecyl Sulfate)?
1 answer-
The Amicon® Ultra centrifugal devices are designed for processing biological fluids and aqueous solutions. Amicon® Ultra Filter Devices are compatible with concentrations of Sodium dodecyl sulfate (SDS)of 0.1% or less. Prior to use, it is important to verify the chemical compatibility of the sample with the device.
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Hi, Do these filters come with expiry dates? If so where would I be able to find them on the product?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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How long should I centrifugate at 14.000 g and how long for the reverse spin at 1000 g?
1 answer-
Typical concentration time for the 3kD membrane device is approximately 30 minutes. However, spin times may need to be adjusted to either a shorter or longer time depending on the sample starting volume, initial concentration, composition, and desired minimum volume. The reverse spin is 1000g for 2 minutes. Please review the product user guide at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/340/pr05485-rev0219.pdfHelpful?
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Hi, how many g's can be used for the ultracell-3K #UFC500396 product?
1 answer-
The recommended speed with a 35 degree fixed angle rotor is 14,000 g with a reverse spin of 1000 g. This information can be found at this link: https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#ultracentrifugal
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