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SCT071

Sigma-Aldrich

BioTracker BacGO Gram-positive Bacteria Dye

Live cell imaging fluorescent probe for detection of gram-posotive bacteria in vitro, in vivo and in bacterial sludge

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1 MG
€161.00

€161.00

Estimated to ship on11 February 2025Details

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1 MG
€161.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

€161.00

Estimated to ship on11 February 2025Details

form

lyophilized

application(s)

cell analysis

detection method

fluorometric

General description

Gram staining has been the gold standard technique for bacterial classification based on the different physical properties of the bacterial cell‐wall structure. However, Gram staining requires several steps, that is, fixation of the bacterial cell, two steps of staining with two different dyes, followed by decolorization, and washing. Notably, the decolorization step often generates false Gram determination, and the washing step can cause significant cell loss. An alternative to the Gram staining method is a fluorescence‐based dye approach for flow cytometry or fluorescence microscopy.BioTracker BacGO Gram-positive Bacteria Dye is an orange colored dye that can be used to identify Gram-positive bacteria by flow cytometry and fluorescence imaging. BacGO dye has been tested using real samples of colony‐forming bacteria, activated bacterial sludge, and a bacterial eye infection model. To fully demonstrate the universal selectivity of BacGO dye, it was tested on 16 bacterial strains (9 Gram‐positive and 7 Gram-negative) and compared the results with Gram staining. BacGO stained all of the known Gram‐positive bacteria and the result was validated with Gram staining. The universal selectivity of BacGO was also confirmed by flow cytometry using DAPI to stain the nuclei of all the bacteria.Spectral properties:Excitation: 558 nmEmission: 573 nmResearch Category:Live Cell Dye

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Pour préparer une solution stock, dans quel solvant/tampon cela doit être fait. Le produit peut-il être congelé une fois en solution. Doit-on l'aliquoter ou peut-il subir plusieurs cycles de congélation-décongélation. 

    1 answer

    1. This product may be reconstituted in anhydrous DMSO. Add 20.6 uL of DMSO to the 1 mg vial to achieve a 100 uM solution. The stock solution can be stored at -20°C for 18 months without loss of activity, as single working aliquots. Avoid repeated freeze/thaw cycles.

      Stock solutions of the dye can be further diluted in a culture medium. Working concentration ranges from 1- 5 uM.
      The incubation period is temperature dependent and may range from 5 minutes for cultures at 37°C to 1 hour for cultures at 4°C.

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