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S2GPT05RE

Millipore

Millipore® Steritop® Vacuum Bottle Top Filter

pore size 0.22 μm, polyethersulfone membrane, funnel capacity 500 mL, pack of 12 ea

Synonym(s):

SCGPT05RE, Steritop® QR Quick Release Bottle Top Filter, bottle top sterile filter unit, bottle top vacuum filter, bottle top vacuum filtering unit

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12 UNITS
€166.00

€166.00

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12 UNITS
€166.00

About This Item

UNSPSC Code:
41104914
eCl@ss:
32031623
NACRES:
NB.24
pore size:
0.22 μm pore size
H:
148 mm
capacity:
500 mL
diam.:
12.5 cm
disc diam.:
73 mm
filter diam.:
73 mm
filtration area:
40 cm2
funnel capacity:
500 mL
material:
polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing
manufacturer/tradename:
Steritop®

€166.00

In StockDetails

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material

polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing

Quality Level

400

sterility

sterile; irradiated

product line

Steritop®

feature

hydrophilic

packaging

pack of 12 ea

manufacturer/tradename

Steritop®

parameter

45 °C max. temp.

description

2.4 mL (hold-up volume)

technique(s)

sterile filtration: suitable

H

148 mm

capacity

500 mL

diam.

12.5 cm

disc diam.

73 mm

filter diam.

73 mm

filtration area

40 cm2

funnel capacity

500 mL

matrix

Express PLUS™

pore size

0.22 μm pore size

fitting

inlet funnel
45 mm outlet thread(s)

shipped in

ambient

General description

This item is a direct replacement for SCGPT05RE
Work with ease and filter with confidence with Stericup® and Steritop® Filtration Systems.

Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.

This Steritop® 500 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.

Application

For Research Use Only
Stericup® and Steritop® systems are designed to reduce foaming and protein denaturation while maximizing flow and can be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.

  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions

Features and Benefits

Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.

Legal Information

EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
STERITOP is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

T Cell Expansion and Viability Using Stericup®-filtered Media

Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.

Protein Binding in Sterile Filtration of Cell Culture Media

Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.

Related Content

Filter Membrane Selection Recommendations

What are you filtering out? What do you want to keep? Pick the right membrane for your filtration.

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Stem Cell Testing of PES Membrane Containing Stericup® Quick Release Filters

This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.

Questions

1–10 of 15 Questions  

  1. What is the expiration date of Stericup® and Steritop® filters?

    1 answer

    1. Typically 3 years from sterilization.

      Helpful?

  2. Can Stericup® and Steritop® filters be reused?

    1 answer

    1. All Stericup® and Steritop® filters were developed for single use, disposable processing.

      Helpful?

  3. What is the chemical compatibility of the various Stericup® and Steritop® filters?

    1 answer

    1. Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf

      Helpful?

  4. What is the maximum vacuum pressure for filtration?

    1 answer

    1. 700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)

      Helpful?

  5. What Bacterial Endotoxins Test is used?

    1 answer

    1. The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.

      Helpful?

  6. Can Stericup® bottles be placed inside freezers for long-term storage of media?

    1 answer

    1. You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.

      Helpful?

  7. What leachables are detected during filtration processes using Stericup® filters?

    1 answer

    1. We have not performed extractables/leachables studies for our Stericup® filters.

      Helpful?

  8. What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?

    1 answer

    1. All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
      *Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.

      Helpful?

  9. Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?

    1 answer

    1. No, all Stericup® and Steritop® devices are Research Use Only.

      Helpful?

  10. What tubing can be used with Stericup® and Steritop® vacuum filters?

    1 answer

    1. Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004

      Helpful?

1–10 of 15 Questions  

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