MABF3366

Anti-Adenovirus 5 DBP Antibody, clone B6-8

• Synonym(s): DBP, DNA-binding protein, Early 2A protein, Early E2A DNA-binding protein
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2A6, monoclonal
• mol wt: calculated mol wt 59,14 kDa; observed mol wt ~~70 kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: Unknown
• Protein ID accession no.: AP_000213
• UniProt accession no.: P03265
• storage temp.: 2-8°C
• Gene Information: vaccinia virus ... DBP(1460850)

Description

Specificity

Clone B6-8 is a mouse monoclonal antibody that specifically detects DNA-binding protein of human Adenovirus 5.

Immunogen

Full length, recombinant DNA-binding protein of human Adenovirus 5.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells infected with human Adenovirus 5.

Western Blotting Analysis (WB): A 1:10,000 dilution from a representative lot detected DNA-binding protein of Adenovirus 5 in lysate from HeLa cells infected with human Adenovirus 5, but not in lysate from uninfected HeLa cells.








Tested Applications

Western Blotting Analysis: A representative lot detected Adenovirus 5 DBP in Western Blotting applications (Andrade F. et al. (2007) EMBO J. 26; 2148-2157).

Immunofluorescence Analysis: A representative lot detected Adenovirus 5 DBP in Immunofluorescence applications (Charman M. et al. (2023) Nature 616(13); 332-368).

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected DNA-binding protein of Adenovirus 5 in lysate from HeLa whole cell extract infected with HAdV-C5-WT, but not in lysate from uninfected HeLa whole cell extract.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

DNA-binding protein (UniProt: P03265; also known as DBP, Early 2A protein, Early E2A DNA-binding protein) is encoded by the DBP gene (Gene ID: 1460850) in human Adenovirus 5. Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. DNA-binding protein (DBP) is a viral component absolutely required for viral DNA replication. It plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion and thus employing its capacity to form multimers. It also enhances the rate of initiation. DBP influences the transcription of viral genes by interacting with viral promoters and enhancers. The expression of DBP is tightly regulated during the viral life cycle and is expressed early in the infection process, following the expression of immediate early genes. DNA-binding protein is mainly localized in the nucleus and which is consistent with its role in viral DNA replication and transcription regulation. DBP contains a short binding region (C-terminal arm; aa 513-529) that bridges DBP molecules together and creates a chain. It homomultimerizes on viral ssDNA bound to pTP and forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1. DBP can interfere with host immune responses, aiding in immune evasion and contributing to the persistence of the virus in the host. Studies show that in adenovirus-infected cells in which gzmB and downstream apoptosis pathways are inhibited, an orphan granzyme gzmH directly cleaves the adenovirus DNA-binding protein (DBP) and induces an important decay in viral DNA replication. (Ref.: Charman, M., et al. (2023). Nature 616(13); 332-363; Andrade, F., et al. (2007). EMBO J. 26, 2148-2157).

Physical form

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable