Yes, the same OPN antibody pair is used in all of the Human Milliplex kits (HANG2MAG-12K, HBNMAG-51K, HCCBP1MAG-58K, HKI4MAG-99K, HND3MAG-39K).
HBNMAG-51K
MILLIPLEX® Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay
The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.
Synonym(s):
human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 82-97%
standard curve range: 0.24-1,000 pg/mL
(TNFα)
standard curve range: 1-3,000 pg/mL
(IL-1β)
standard curve range: 1-6,000 pg/mL
(ACTH & IL-6)
standard curve range: 146-600,000 pg/mL
(OC)
standard curve range: 24-100,000 pg/mL
(SOST)
standard curve range: 37-150,000 pg/mL
(FGF-23)
standard curve range: 49-200,000 pg/mL
(Leptin)
standard curve range: 5-20,000 pg/mL
(DKK-1)
standard curve range: 5-20,000 pg/mL
(PTH)
standard curve range: 61-250,000 pg/mL
(Insulin)
standard curve range: 7-30,000 pg/mL
(OPG)
standard curve range: 98-400,000 pg/mL
(OPN)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.
MILLIPLEX® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Bone
MILLIPLEX® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Bone
Specificity
Cross Reactivty
No cross reactivity between the antibodies for an analyte in this panel were observed.
No cross reactivity between the antibodies for an analyte in this panel were observed.
Application
- Analytes: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
- Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or extracts
- Recommended Sample Dilution: 25 μL per well of d1:2 diluted serum or plasma; cell/tissue culture samples may be used neat or diluted in appropriate control medium
- Assay Run Time: Overnight (16–18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
- Research Category: Metabolism
- Research Subcategory: Bone
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Linkage
Replaces: HBN1B-51K
Other Notes
Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
A novel fluorescent probe-based flow cytometric assay for mineral-containing nanoparticles in serum.
Edward R Smith et al.
Scientific reports, 7(1), 5686-5686 (2017-07-20)
Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are indirect, lack
Sharon M Moe et al.
Circulation, 132(1), 27-39 (2015-06-11)
Patients with kidney disease have disordered bone and mineral metabolism, including elevated serum concentrations of fibroblast growth factor-23 (FGF23). These elevated concentrations are associated with cardiovascular and all-cause mortality. The objective was to determine the effects of the calcimimetic cinacalcet
Nigel Kurgan et al.
Frontiers in endocrinology, 11, 531926-531926 (2020-12-29)
In adults, excess adiposity has been associated with low-grade, chronic inflammation and compromised bone health, but less is known about these linkages in children. The purpose of this study was to compare the circulating levels of inflammatory cytokines, adipokines, osteokines
Chiara Giannasi et al.
International journal of medical sciences, 16(1), 23-32 (2019-01-22)
Bisphosphonates (BPs) represent the first-line treatment for a wide array of bone disorders. Despite their well-known action on osteoclasts, the effects they induce on osteoblasts are still unclear. In order to shed light on this aspect we evaluated the impact
Fawaz Y Azizieh et al.
Biomarker insights, 14, 1177271919843825-1177271919843825 (2019-08-28)
Receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and oxidative stress markers are suggested to contribute to bone loss in osteoporosis that occurs in menopause. However, the association between these markers and bone mineral density (BMD) is controversial.
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Are the OPN antibodies used for detection and capture the same in all Human Milliplex kits?
1 answer-
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What is the specificity of Osteocalcin in the Human Bone Panel, HBNMAG-51K?
1 answer-
The Osteoclacin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated in the appropriate positions for each species. These antibodies were then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, it is unknown whether the antibodies recognize non-carboxylated or under-carboxylated material, or only carboxylated material, as this has never been tested.
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How can I determine if the Human Bone Milliplex kit is measuring total OC or uncarboxylated OC?
1 answer-
The osteocalcin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated and then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, the antibodies have not been tested to determine if they recognize non-carboxylated, under-carboxylated, or only carboxylated material.
For the Human Bone panel measuring Osteocalcin, the capture antibody is a monoclonal OC antibody that recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. The detection antibody, on the other hand, is a monoclonal OC antibody that also recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 1-19, 7-19, and 15-31 of the native molecule.
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